Nanoglo luciferase assay system, promega, promega vwr. Luciferase reporter gene assay principle kaboom latam inc. Soda pdf is the solution for users looking to merge multiple files into a single pdf document. A smaller donor reduces the problem of steric effects when studying protein. Generally, 100fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase cat assay.
Select or drag your files, then click the merge button to download your document into one pdf file. Buy dual luciferase reporter assay system reporter assays, promega model pae1960 each on free shipping on qualified orders. For an overview and information on the development of the dualglo luciferase assay system, see sections 6. Luciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Description firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening1, 2. Can be used for assays in singletube luminometers or in multiwellplate luminometers with autoinjectors.
Product components and storage conditions product size cat. Jan 29, 20 see also promega dualluciferase reporter assay system as well as promega pgl3 luciferase reporter vectors available from promega corporation, madison, wis. Instructions for use of products e2920, e2940, e2980. Promega e4530 luciferase assay system freezer pack. In order to develop a highthroughput exoerythrocyticstage malaria assay capable of screening large libraries of chemical compounds, a number of tests were performed to optimize a 48 h in vitro pbgfplucsm con 19 infection of hepg2a16cd81 egfp hepatocytes 20.
Luciferase assay systems instructions for use of products e1483, e1500, e1501, e1531, e4030, e4530 and e4550. The nanoglo luciferase assay system provides a simple, singleaddition reagent that generates a glowtype signal in the presence of nanoluc luciferase with a halflife of approximately 120 minutes in commonly used tissue culture media. General considerations the nanoglo dualluciferase reporter assay system is designed to be used with many media types and has been validated for use with the following culture media containing 010% serum. The dualglo luciferase assay system is designed to allow highthroughput analysis of mammalian cells containing genes for firefly and renilla luciferases, grown in 96 or 384well plates.
Elisa, western blotting, dot blotting, immunogen, protein array, function assay, etc. Promega has launched the new nanoglo dualluciferase reporter nanodlr assay, a tworeporter system that incorporates nanoluc luciferase technology, providing increased data quality and greater sensitivity for biologically complex applications. The nanoglo dualluciferase reporter assay system is designed to be used with many media types and has been validated for use with the following culture media containing 010% serum. The nanodlr assay allows researchers to measure nanoluc and firefly luciferases together and its chemistry provides improved reagent. I performed a dual luciferase assay using pgl3 firefly luc as experimental reporter and prltk renilla luc as internal control promega assay kit. To see articles that cite the use of the dualglo luciferase assay system, visit. May 31, 2012 promega corporation announces the launch of a novel luciferase that is smaller, brighter, and more versatile than any current bioluminescent enzyme. The comparison conventional assay, which is an adaptation of the assay published in the first reports of luciferase used as a reporter of genetic events, such as transcription, mol. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Sensitivity contains alla commonly used reporter genes.
Easily combine multiple files into one pdf document. Multiplexing luciferase reporter and cell viability assays. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 20 moles of luciferase can be measured under optimal conditions. General considerations the steadyglo luciferase assay system is designed for use with the following culture media containing 010% serum. Nanoglo dualluciferase reporter assay nanodlr system. Linear results are seen over at least 8 orders of magnitude of enzyme concentration, and the patented technology incorporated into this assay system allows measurement of less than 1020 moles of luciferase under optimal conditions. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Create and merge pdfs with pdfcreator and let pdf architect help you edit pdfs, insert images to pdfs, extract text from images and more. The dualglo luciferase reagent can be added directly to cells in growth medium without washing or preconditioning. The nanodlr assay reagents should give a signal halflife of approximately 2 hours at 22c for.
The nanodlr assay allows researchers to measure nanoluc and firefly luciferases together and its chemistry provides improved reagent stability over. Promega has launched the new nanoglo dual luciferase reporter nanodlr assay, a tworeporter system that incorporates nanoluc luciferase technology, providing increased data quality and greater sensitivity for biologically complex applications. Promega corporation, which are incorporated by reference in their entirety. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Apr 05, 2019 because of this, a fireflyrluc system should be measured using the dualglo luciferase assay system e2920 or dualluciferase reporter assay system e1910. Watch the subtitled version of the video here continue reading from live cells to lysates. Performing the nanoglo dual luciferase reporter assay 3. In the dlr assay, the activities of firefly photinus pyralis and renilla renilla reniformis, also known as sea pansy luciferases are measured sequentially from a single sample. Control reactions are monitored easily by a luciferase assay for the production of luminescence generated from the fulllength luciferase.
In a pinch, all three of the dual kits can be used to measure firefly luminescence, but the second reporter must match the kit. Our expert industry analysis and practical solutions help you make better buying decisions and get more from technology. Promega has provided a suite of nanoluc fusion vectors to enable intracellular protein stability assays. Apr 14, 2015 promega corporation has announced the launch of the new nanoglo dualluciferase reporter nanodlr assay, a tworeporter system that incorporates nanoluc luciferase technology, providing increased data quality and greater sensitivity for biologically complex applications. Buy dualluciferase reporter assay system reporter assays, promega model pae1960 each on free shipping on qualified orders. Luciferase assay to study the activity of a cloned promoter. It is a very sensitive genetic reporter due to the lack of any endogenous activity in mammalian cells or tissues3, 4. Luciferase assay to study the activity of a cloned. Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. In our experience, 50 luciferase assays can be performed in about 1 hour. Instructions for use of products g7570, g7571, g7572, g7573.
Merge pdf online combine pdf files for free foxit software. In summary, the crebased luciferase assay was suitable to monitor the activation of three different 5ht receptors, and can be easily adapted for compound screening. Thanks to this pdf merger you can easily merge combine files. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Promega dualglo luciferase assay system a homogeneous reagent system that enables fast, simple quantitation of both firefly and renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The experimental setup was similar for each assay combination. Resuspend the lyophilized luciferase assay substrate in luciferase assay buffer ii 10ml for cat. Promegas luminescent celltiterglo assay 2 was multiplexed with either the luminescent enduren live cell substrate, or the luminescent viviren live cell substrate. Luciferase reporter gene assay principle format ogg transformation and transfection assays gene in thiselement.
Promega corporation announces the launch of a novel luciferase that is smaller, brighter, and more versatile than any current bioluminescent enzyme. After using the dual luciferase assay from promega, however, i am getting luminescence values from 300 to 1200 for firefly luciferase and 1530 for renilla luciferase background is 12. In this process, substrate proteins are covalently linked to multiple ubiquitin molecules, which target the protein to the 26 s proteasome for degradation. Luciferase reporter gene assay on human 5ht receptor.
How to merge pdfs and combine pdf files adobe acrobat dc. Ismail has translated the assay into a cellfree, biochemical format, termed the nanobit biochemical assay nbba. The signal halflife under these conditions is greater than 5. Page 4 revised 7 steadyglo luciferase assay system protocol 3. The luciferase assay system was developed for reporter quantitation in mammalian cells. Ideally suited for high and ultrahighthroughput applications, the oneglo assay contains a new luciferase substrate, resulting in a reagent that is more stable, more. Combine multiple pdf files into one pdf, try foxit pdf merge tool online free and easy to use. An extremely sensitive reagent for rapid quantitation of firefly luciferase. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 phone 6082744330 fax 6082772516. Promega luciferase assay systems an extremely sensitive reagent for rapid quantitation of firefly luciferase. The firefly luciferase is a 62,000 dalton protein, which is active as a monomer and does not require subsequent processing for its activity.
Dualluciferase reporter assay system protocol promega. On the top of that this merge pdf files is really fast, free, lightweight and stable. Celltiterglo luminescent cell viability assay protocol. The oneglo luciferase assay system provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. The celltiterglo assay is designed for use with multiwell plate formats, making it ideal for automated highthroughput screening hts, cell proliferation and cytotoxicity assays. Add 1 volume of 5 a siv ey u f r to 4 volumes of distilled water. These target sites can be introduced by cloning putative mirna binding sites alone, or the 3. Studying protein interactions with bret and nanoluc. The 26 s proteasome is a 2mda multisubunit complex that degrades proteins in an atpdependent manner into peptides 320 amino. Does someone have experience with promega nanoluc luciferase.
Measuring intracellular protein lifetime dynamics using. These vectors facilitate subcloning of nterminal and cterminal fusions with nanoluc luciferase using standard restriction enzyme cloning or promegas convenient flexi cloning system table 2. Jun 24, 1997 the comparison conventional assay, which is an adaptation of the assay published in the first reports of luciferase used as a reporter of genetic events, such as transcription, mol. We suggest that the crebased luciferase assay may be also useful for detecting other 5ht receptors and is beneficial for discovering novel 5ht receptor ligands. The gold standard 51 crrelease assay is very accurate but has the major. Highthroughput luciferasebased assay for the discovery.
Luciferase based assays have become an invaluable tool for the analysis of cloned promoter dna fragments, both for verifying the ability of a potential promoter fragment to drive the expression of a luciferase reporter gene in various cellular contexts, and for dissecting binding elements in the promoter. Bmg labtechs highend multimode plate reader pherastar is used to perform multiplexed luciferase reporter and cell viability assays. A free and open source software to merge, split, rotate and extract pages from pdf files. Allard and kevin kopish, promega corporation luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of use when investigating questions that involve gene regulation. Pdf merge combine pdf files free tool to merge pdf online. Ideally suited for high and ultrahighthroughput applications, the oneglo assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor. The luciferase assay system yields linear results over at least eight orders of magnitude. Technical manual dual luciferase reporter assay system instructions for use of products e1910 and e1960. Promega dualglo luciferase assay system fisher scientific. The homogeneous assay procedure involves adding a single reagent celltiterglo reagent directly to cells cultured in serumsupplemented medium. Dualluciferase reporter assay systems instructions for use of products e1910, e1960 and e1980.
Luciferase mrna transfection of antigen presenting cells. Dualluciferase reporter assay system harvard university. Luciferase control rna is an uncapped in vitrotranscribed rna containing a 30base polya tail that produces functional luciferase when translated. The degradation of most proteins in mammalian cells occurs via the ubiquitinproteasome pathway.
Less than 10 20 moles of luciferase have been detected under optimal conditions. Promega s multiplexed luciferase reporter and cell viability. It is an extremely easy, blazing fast and highly secure app to merge and split your pdf document. Luciferase assay system, 10pack e1501 from promega.
The dualluciferase reporter dlr assay system provides an efficient means of performing dualreporter assays. Development of a luciferasebased highthroughput exoerythrocyticstage assay. Pdf merger, combine pdf files into one file online. Promega s multiplexed luciferase reporter and cell. The luciferase assay system is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. The system contains sufficient reagents to perform 1,000 luciferase assays 100l per assay.
With the emerging fields of cancer vaccines and adoptive cell transfer therapies, there is an increasing demand for highthroughput in vitro cytotoxicity assays that efficiently analyze immune effector functions. Promega luciferase assay systems fisher scientific. Cellular assays have always been a powerful tool in the research lab. This free online tool allows to combine multiple pdf or image files into a single pdf document. In contrast, the standard cat and neo assays require laborious extractions, chromatography, or gel separations 5,6,7. New promega luciferase is smaller, brighter and more versatile. They are incredibly versatile, and can be designed to measure virtually any cellular or. These attributes of the nanoluc luciferase provide new capabilities in reporter assays with potential in biologically complex applications that require greater sensitivity. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single and dualspectral analyses. For kits e1500, e1501, e4030, e4530 and e4550, prepare luciferase assay reagent lar by adding luciferase assay buffer 10ml for e152a and 100ml for e152b to the vial of lyophilized luciferase assay substrate. The pmirglo dualluciferase mirna target expression vector is designed to quantitatively evaluate microrna mirna activity by the insertion of mirna target sites 3. The dualglo luciferase assay system is designed to work in growth media commonly used for mammalian cells with or without added serum.
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